Background: 2,3-Butanediol (2,3-BD), a valuable compound used for chemicals, cosmetics, pesticides and pharma\u2011 ceuticals, has been produced by various microbes. However, no high-temperature fermentation of the compound at high productivity has been reported.<\/p>\n
Methods: Thermotolerant xylose-utilizing microbes were isolated from 6 diferent districts in Laos and screened for a low accumulation of xylitol in a xylose medium at 37 \u02daC. One isolate was found to produce 2,3-BD and identifed by 16S rDNA sequencing. The 2,3-BD fermentation capacity was investigated at diferent temperatures using xylose and glucose as carbon sources, and the fermentation parameters were determined by a high-performance liquid chroma\u2011 tography system<\/p>\n
Results: By screening for a low accumulation of xylitol in a xylose medium, one isolate that accumulated almost no xylitol was obtained. Further analyses revealed that the isolate is Cronobacter sakazakii and that it has the ability to produce 2,3-BD at high temperatures. When xylose and glucose were used, this strain, named C. sakazakii OX-25, accumulated 2,3-BD in a short period before the complete consumption of these sugars and then appeared to con\u2011 vert 2,3-BD to acetoin. The optimum temperature of the 2,3-BD fermentation was 42 \u02daC to 45 \u02daC, and the maximum yield of 2,3-BD was 0.3 g\/g at 12 h in 20 g\/l xylose medium and 0.4 g\/g at 6 h in 20 g\/l glucose medium at 42 \u02daC. The 2,3-BD productivity of the strain was higher than the 2,3-BD productivities of other non-genetically engineered microorganisms reported previously, and the highest productivity was 0.6 g\/l\u00b7h and 1.2 g\/l\u00b7h for xylose and glucose, respectively.<\/p>\n
Conclusions: Among thermotolerant microbes isolated in Laos, we discovered a strain, C. sakazakii OX-25, that can convert xylose and glucose to 2,3-BD with high efciency and high productivity at high temperatures, suggest\u2011 ing that C. sakazakii OX-25 has the potential for industrial application to produce 2,3-BD as an important platform chemical.<\/p>\n","protected":false},"template":"","meta":{"_acf_changed":false},"access-right":[],"featured":[100],"language":[152],"province":[185],"resource-type":[144],"topic":[155,164],"class_list":["post-13570","resource","type-resource","status-publish","hentry","featured-yes","language-english","province-vientiane-capital","resource-type-research-article","topic-agroforestry-and-ecosystem-management","topic-health-and-nutritional"],"acf":[],"_links":{"self":[{"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/resource\/13570","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/resource"}],"about":[{"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/types\/resource"}],"wp:attachment":[{"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/media?parent=13570"}],"wp:term":[{"taxonomy":"access-right","embeddable":true,"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/access-right?post=13570"},{"taxonomy":"featured","embeddable":true,"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/featured?post=13570"},{"taxonomy":"language","embeddable":true,"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/language?post=13570"},{"taxonomy":"province","embeddable":true,"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/province?post=13570"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/resource-type?post=13570"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/phakhaolao.la\/en\/wp-json\/wp\/v2\/topic?post=13570"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}